Study of neuronal circuit dynamics from multiple brain or spinal cord regions
Chromatone™ is a multiscope™ for simultaneous multi-region, multi-color stimulation and neuronal imaging in freely behaving animals, accompanied by its software package.
- Allows functional imaging of neuronal circuits with optogenetic manipulation in up to four brain or spinal cord areas.
- Enables the simultaneous recording of neurons at cellular resolution.
- Capabilities of Quartet® and more.
Included with purchase:
- Hands-on-surgical training
- Neuronal trace extraction support
- Device synchronization with other behavior equipment
GFP & RFP Imaging and Channelrhodopsin & ChrimsonR Optogenetic Stimulation
- Power level emitted for each wavelength can be independently controlled in each imaging fiber optics.
- Optogenetics protocols can be set by users independently and trigger off to perform behavioral tasks.
Specifications
| Illumination colors in each fiber optic | 470 +/- 12 nm (GFP, Channelrhodopsin optogenetics) 550 +/- 7.5 nm (RFP) 632 +/- 14 nm (ChrimsonR optogenetics) |
| Illumination power range at tissue | 470 nm: 0 to 6 mW 550 nm: 0 to 1 mW 632 nm: 0 to 2 mW |
| Illumination mode for each wavelength | Green: CW for imaging (can be pulsed, if required) |
| Blue: Pulse, can get triggered and executes the Optogenetics protocol set by user | |
| Red: Pulse, can get triggered and executes the Optogenetics protocol set by user | |
| Optical Resolution | 7 µm |
| Focusing Range or Working Distance | 250 µm in brain tissue |
| Number of Imaging Probes | 4 |
| Diameter of GRIN Lens to Implant | Customizable between 0.5 mm to 1 mm |
| Available Length of Lens to Enter Brain Tissue | Customizable between 2.5 mm to 8 mm |
| Field of View in Each Region | Approximately the same as the lens diameter |
| Weight of Connector and Lens | < 400 mg |
| Approx Weight of each Fibre Bundle Carried by Animal | < 1 g |
| Bending Radius of Fiber Bundles | 3 cm |
| Length of Imaging Fiber Bundles | 84 cm |
| Detection Wavelength | 517 +/- 13 nm and 590 +/- 20 nm |
| Image Size per Frame | 3.2 megapixels |
| Number of Frames per Second | 20 in continuous mode, 140 in burst mode |
| Length of Camera Electric Wire | 2 meters |
| Length of LED Wire | 1.5 meter |